Introduction to Single-Particle Cryo-EM - Astbury Centre
Training
Date: 01-Sep-2025 to 04-Sep-2025
Instruct Partner
This in-person beginners’ course is aimed at PhD students and postdoctoral researchers who are new to cryo-electron microscopy (cryo-EM) and plan to incorporate it into their research. The programme will combine core theoretical lectures with practical training in single-particle cryo-EM, covering:
- Negative stain grid preparation and screening
- Cryo-EM sample preparation (including sample preparation for time-resolved cryo-EM)
- Grid screening and data collection using EPU on a Titan Krios microscope
- Demonstration of single-particle data processing using RELION
- Participants are encouraged to send their samples in advance (one sample per attendee) for screening and potential data collection during the practical sessions. Samples must be received no later than 15 August 2025 and will be subject to quality control. A limited number of samples will be accepted.
Key Details
- Dates: 1–4 September 2025
- Location: The Astbury Biostructure Laboratory, University of Leeds, UK
- Capacity: 18 participants
- Registration Deadline: 17 July 2025
- Course Fee: Free of charge
- Included: Accommodation (check-in 31 August, check-out 4 September), meals, and travel reimbursement up to £250. Participants are responsible for any costs exceeding this amount.
- Find more information and register here
Applicants must meet the following criteria:
- Be a PhD student or postdoc new to the field and will be using cryo-EM as part of your work
- Have access to the necessary cryo-EM instrumentation at your home institution or through a local facility, or have established plans to secure such access in the near future.
The Astbury Centre, University of Leeds, is part of Instruct-UK. The facility offers access to cryo-EM, solution NMR, and high-resolution native mass spectrometry through Instruct. Find out more about The Astbury Centre and apply to access the facility here.
