View All Protein Production at Instruct
The core facility for protein production provides services covering DNA cloning and DNA plasmid preparation, protein expression in E. coli expression systems and recombinant protein purification. Our services can also include optimization of expression and purification protocols, protein identity and purity testing and determination of protein concentration.
Cloning includes primer design, preparation of DNA plasmids for protein expression using either traditional restriction enzymes or restriction-free methodology, mutagenesis of existing plasmids, and ensuing control by sequencing.
Literature research, project optimization, cloning design
Expression of proteins in Escherichia coli. Procedure includes preparation of media, inoculation of over night culture, induction of expression in 1 L batches and separation of cell pellet from media by centrifugation.
Recombinant protein purification
