InstructCryo-Electron Microscopy (CryoEM) has become a well-established technique for determining the three-dimensional structures of biological macromolecules. It currently achieves atomic resolution and enables the study of conformationally heterogeneous samples as well as macromolecular complexes. Cryo-EM complements other structural techniques such as X-ray crystallography and Nuclear Magnetic Resonance and is currently the fastest-growing technique in atomic structure depositions in public databases like the PDB.
The aim of this course is to introduce participants to the analysis of conformational flexibility/heterogeneity, a crucial step in Cryo-EM image processing to obtain dynamic information in the form of conformational landscapes. The I2PC has extensive experience in organising courses on this topic, with previous editions being in high demand. The course is organised and run by the Instruct Image Processing Centre (I2PC) part of CNB-CSIC in Madrid.
This course is intended for researchers at all levels.
The goal is to introduce researchers to flexibility/heterogeneity analysis, one of the most advanced areas in Single Particle Analysis (SPA). The course will cover the theory behind the most commonly used methods in the field and include hands-on practical sessions within the Scipion Flexibility Hub environment, facilitating the use of multiple tools within a single workflow.
Flexibility analysis in Cryo-EM is an emerging technique that enables the capture of different conformations of studied macromolecules. Gaining expertise in this area provides an added value for researchers with prior experience in Cryo-EM image processing, enhancing their analytical capabilities and understanding of the structural dynamics of biomolecules.
Tutors
Marcos Gragera (MG) – Single Particle Analysis specialist, BCU/I2PC
David Herreros (DH) – Flexibility Hub head developer, BCU
Carlos-Oscar Sorzano (COS) – Group leader, BCU; Technical Director, I2PC
Slavica Jonic (SJ) – Research Director, CNRS
